Abstract: Culture is the cornerstone where societies have always supported their respective identities. It is a way of life, as long as it expresses not only the values, but also the education, the art, and the daily life of all those who live and work in a society. Thessaloniki has been a multicultural city, gathering populations from different nations and religions over time. The city was a refuge destination for many persecuted Jews in Europe, between 1492 and 1943, which led to the creation of a large Jewish community. As a result, the long tradition combined with the several elements of Jewish cultural interest attracts a large number of tourists from Israel every year. This paper presents the main research results conducted in Thessaloniki regarding tourists’ from Israel motivations and characteristics, but also their main elements of interest. Thessaloniki has all the essential elements, such as history, tradition, monuments, infrastructure, and services for the development of cultural tourism. These, however, need to be improved, so that the city of Thessaloniki becomes a more popular tourist destination for Israelis.